Learners view video clips demonstrating acid-fast staining procedures. Correctly stained slides are shown. The learning object includes two matching exercises.
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This learning object shows how the shape of a stain defines the angle of impact. In general terms the more circular the stain, the more perpendicular will be the angle at which it struck the surface. The more elliptical the shape of the stain, the more acute the angle will be. With practice and experience, the analyst can recognize the general angle of impact based solely on the shape of the stain.